We describe a novel technique to perform whole-body perfusion fixation in mice with specific relevance to micro-imaging. With the guidance of high-frequency ultrasound imaging, we were able to perfuse ...

For histological investigations of biological tissues, i.e. anatomical studies under the microscope, samples are usually fixated to prevent them from decaying. Fixation is typically done by immersing ...

Collect tissues using sharp scissors or scalpel blades and mouse-tooth forceps. Handle tissues gently. Grasp and cut samples at the edges, so the areas of interest aren't damaged. At right is a ...

Researchers at Kanazawa University report in Communications Biology that using common chemicals for fixing living cell samples for microscopy studies causes membrane proteins to aggregate. The ...

Animal perfusion is important for a plethora of operations including multiple in vivo imaging modalities and targeted gene/drug delivery. Perfusion takes the right reagents, the right parameters, and ...

Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

1. In vitro experiments with nucleic acid impregnated agar blocks, using four different types of fixatives, show that the Feulgen nuclear stain gives two types of staining curves, depending on the ...

Calcium (Ca)-containing fixation solutions applied to slices of electric organ of the electric ray, Narcine brasiliensis, have been shown to have three distinct ultrastructural effects on cholinergic ...