The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...
Integrated DNA Technologies (IDT) has launched a new and enhanced version of the open access PrimerQuest® online tool, for efficient PCR primer and probe design. Selection parameters have been ...
Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...
The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...
GeneFisher—fishing for genes, perhaps? Fishing genes out of genomes? Just fishing? The subtitle, Interactive PCR Primer Design, “hooked me” (get it?) but it shouldn’t have in retrospect. After all, ...
Genome editing using designer nucleases such as transcription activator-like effector nucleases (TALENs) or clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 nucleases is an ...
Houston, April 19, 2022 (GLOBE NEWSWIRE) -- NuProbe, a global genomics and molecular diagnostics company, announces its recent publication in Nature Communications describing a massively multiplexed ...
Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today announced the launch of an assay website that allows droplet digital PCR (ddPCR™) users to locate existing assays or design new ones for mutation ...
The polymerase chain reaction (PCR) is a molecular method that is employed for in vitro amplification of a specific region in a strand of deoxyribonucleic acid (DNA). Multiplex PCR involves the ...
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