Direct fibroblast reprogramming: an emerging strategy for …
Innovative methods for fibroblast reprogramming like small molecules, CRISPRa, modified mRNA, and the challenges of cellular heterogeneity and senescence faced by in vivo direct …
High-efficiency RNA-based reprogramming of human primary fibroblasts
Feb 21, 2018 · Here, the authors show highly efficient reprogramming of human primary fibroblasts to iPSCs via the synergistic activity of synthetic modified mRNAs, mature miRNA …
Efficient reprogramming of human fibroblasts using RNA reprogramming …
Dec 1, 2022 · In summary, RNA reprogramming with DAPT/iDOT1L can accelerate reprogramming of human fibroblast cells without decreasing p53 expression, and we showed …
Direct reprogramming of fibroblasts into antigen-presenting …
This study aimed at defining the minimal transcription factor combination necessary to reprogram fibroblasts in functional antigen-presenting DCs. All experiments were repeated at least once.
Competing dynamic gene regulatory networks involved in fibroblast ...
May 13, 2025 · Investigating the gene regulatory networks involved during reprogramming would help generate functional cells in adequate numbers. To address this, we developed an …
Direct reprogramming of mouse fibroblasts into self-renewable …
Jun 23, 2025 · Here, we report the direct reprogramming of mouse tail tips and embryonic fibroblasts into induced pulmonary alveolar epithelial-like cells (iPULs) using four transcription …
RNA-based Reprogramming of Human Primary Fibroblasts into …
Here, we present a clinically relevant, step-by-step protocol for the extremely high-efficiency reprogramming of human dermal fibroblasts into iPSCs using a non-integrating approach.
Engineering fibroblast with reprogramming and spheronization …
Aug 1, 2025 · Here, we present a method to engineer fibroblasts through small molecule reprogramming and spheronization, achieving functional osteoblastic materials across all age …
Direct Reprogramming Improves Cardiac Function and Reverses …
Dec 12, 2022 · In vivo cardiac reprogramming generates new induced cardiomyocytes from resident cardiac fibroblasts, improves cardiac function, and reduces fibrosis in chronic MI in mice.
Fibroblast rejuvenation by mechanical reprogramming and ...
Apr 29, 2020 · In this paper, we describe a unique method of fibroblast rejuvenation, which involves partial reprogramming of fibroblasts by growing them under lateral confinement, …
Rejuvenation capacity of genetic versus human-optimized …
1 day ago · Small-molecule chemical reprogramming is an alternative, yet its performance relative to genetic approaches in human cells is unresolved. We directly compared a human-optimized …
Fibroblast Reprogramming in Cardiac Repair - JACC: Basic to ...
Sep 21, 2023 · This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of …
Fibroblast Reprogramming in Cardiac Repair - PMC
This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of reprogramming …
Direct reprogramming of human fibroblasts into insulin ... - Nature
Mar 24, 2023 · This work provides proof-of-concept of the capacity to make insulin-producing cells from human fibroblasts via transcription factor-mediated direct reprogramming.
Cancer associated fibroblasts and metabolic reprogramming: …
Metabolic reprogramming provides tumors with an energy source and biofuel to support their survival in the malignant microenvironment.
How to reprogram human fibroblasts to neurons - PMC
In recent years, studies on reprogramming human fibroblasts into neurons have emerged one after another, and this paper summarizes all these studies. Scientists find small molecules and …
Identifying regulators of aged fibroblast activation in 3D tissue ...
6 days ago · Robust 3D tissue culture models to activate aged fibroblasts for cell-based therapies and identify regulators of such activation are still missing. In our previous study, we showed …
Fibroblast rejuvenation by mechanical reprogramming and ...
In this paper, we describe a unique method of fibroblast rejuvenation, which involves partial reprogramming of fibroblasts by growing them under lateral confinement, followed by their …
Fibroblast Reprogramming in Cardiac Repair - ScienceDirect
Jan 1, 2024 · This review will discuss recent progress of fibroblast reprogramming in cardiac repair, including different types and states of reprogrammed fibroblasts, optimization of …
Direct fibroblast reprogramming: an emerging strategy for …
Feb 27, 2025 · Fibroblasts are frequently employed in reprogramming studies due to their abundance and accessibility. However, they are also the key drivers in the progression of …
Fibroblast rejuvenation by mechanical reprogramming and ...
May 12, 2020 · Over the course of the aging process, fibroblasts lose contractility, leading to reduced connective-tissue stiffness. A promising therapeutic avenue for functional …
Cancer-Associated Fibroblasts Promote Imatinib Resistance in ...
Nov 21, 2025 · Here, we identified a role for cancer-associated fibroblasts (CAFs) in driving imatinib resistance. Specifically, TGF-β1 secreted by CAFs amplified CCN2/Rack1 signaling.