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Oct 27, 2022 · GLORI is an unbiased, convenient method for the absolute quantification of the m6A methylome. The m6A modification is mapped transcriptome-wide at single-base resolution in …

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GLORI relies on glyoxal and nitrite-mediated deamination of unmethylated adenosines while keeping m6A intact, thereby achieving specific and efficient m6A detection.

Here we provide a detailed protocol for proper RNA sample handling and provide further guidelines for the use of a tailored bioinformatics pipeline (GLORI-tools) for subsequent data analysis.

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