CRISPR-Cas Delivery Systems and Cargo Types ✅CRISPR cargo formats CRISPR genome editing can be delivered in multiple molecular forms. These include Cas9/sgRNA ribonucleoprotein (RNP) complexes for rapid and transient editing, dCas9/sgRNA RNPs for gene regulation, plasmid DNA encoding CRISPR components, and Cas9 mRNA combined with sgRNA for controlled, short-term expression. ✅RNP- and RNA-based delivery RNP complexes and mRNA/sgRNA mixtures enable fast editing with reduced off-target effects and

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CRISPR-Cas9 Off-Target Effects: Challenges and Solutions